Maling Gou
DLP-based in vivo 3D bioprinting
报告人:Maling Gou
所在单位:State Key Laboratory of Biotherapy, Sichuan University
Biography:
Maling Gou, Professor, State Key Laboratory of Biotherapy (SKLB), Sichuan University, China. He received his Ph.D. on Biotherapy of Human Diseases from Sichuan University in 2010 and continued scientific research at SKLB. In 2012, he worked as a visiting scholar in University of California, San Diego (USA) for one year. Besides, he also serves as Associate Editor of Bio-design & Manufacturing and on the Editorial Boards of several international journals including Inter J Nanomed, Int J Bioprint and Burns & Trauma. He has published over 100 peer-reviewed papers in international journals including Nature, Science Advances, Nature Communications, Advanced Science, Advanced Functional Materials, ACS Nano, Small. Meanwhile, he has obtained over 10 licensed Chinese invention patents, part of which have been transferred to enterprise. His work was supported by national projects including the National Science Fund for Distinguished Young Scholars. His research focuses on R&D of 3D bioprinting and nanotechnology for innovative drugs or medical devices.
Abstract:
3D-printing is emerging as a disruptive technology that has great promise for advancing medicine. For clinical medicine, one of the major treads is minimal- or noninvasive. However, the existing application methods of 3D printing technology are limited to ex vivo 3D-printing followed by surgical implantation or in situ 3D printing at the exposed trauma, both of which need exposure of the application site. A novel noninvasive in vivo 3D bioprinting technology enabled by a digital near-infrared (NIR) photopolymerization (DNP)-based 3D printing technology was established. In this process, we use a digital micromirror device (DMD) to modulated near-infrared light (NIR) for 3D-printing. This process was ensured by a newly designed nano-initiator that can convert the NIR to 365 nm light and subsequently efficiently initiate the photopolymerization of commonly used hydrogel monomers. Cells could be readily mixed into the biocompatible aqueous monomer solution for bioprinting of personalized living tissue constructs. In the animal experiments, a personalized ear-like constructs with chondrification was non-invasively printed in vivo, showing potential clinical application. Also, an adipose-derived stem cells-seeded conformal scaffold was non-invasively printed for efficiently promoting the muscle defects repair in vivo. In summary, this work provides the proof-of-concept for the non-invasive in vivo bioprinting, which would open a new avenue for medical 3D printing and advance the minimal- or non-invasive medicine.
