Mingxing Lei
Skin organoid and tissue engineering of hair regeneration
报告人:Mingxing Lei
所在单位:Chongqing University
Biography:
Mingxing Lei serves as a professor at Chongqing University. He is dedicated to studying the regeneration of skin and appendages. He has held 8 Projects supported by the National Natural Science Foundation of China(NSFC), the Chongqing Talents Program etc. He has published over 40 papers in journals such as Science, Cell Stem Cell, PNAS, etc. Several papers were selected as cover articles of journals, or highlighted by media such as CNBC. He was selected as a Chongqing Talent in 2020, and won several awards including the Albert Kligman Travel Award of the 75th Society of Investigative Dermatology Conference.
Abstract:
The transformation process from the dissociated multipotent stem or progenitor cells into an organized tissue is a poorly understood and yet fundamental issue in regenerative medicine. To explore this process, we developed an in vitro transwell 3-dimensional mixed culture, in which dissociated newborn mouse epidermal and dermal cells can self-organize to form planar hairy skin. With more than 500 time-lapse movie segments, we found dissociated cells go through a complex morphogenetic detour to become a planar skin, from small aggregates-dermal/ epidermal cysts-fused cysts-hair-bearing planar skin. Transcriptome analyses by non-biased clustering of molecular profiling data categorize these processes into different phases. In the early phase, adhesion molecules, and morphogens such as PDGF and Notch are more involved. In the late phase, Yap1, Wnts, and extracellular matrix molecules are more involved. Functional perturbations show the timely physical-chemical coupling of these molecular events with phase transition are essential for tissue self-organization. When transplanted to the backs of nude mice, these reconstituted cultured skin grafts robustly formed hairs. While cells from adult mice do not form hairs easily, by comparing transcriptome analyses and cellular events, we made progress toward making adult cells form new hairs. The results demonstrate the self-organizing process from dissociated progenitor cells to planar reconstituted skin with newly induced hair follicles.
