Bio-manufacturing of in vitro cancer models based on diverse biomaterial approaches for molecular mechanism studies报告人:
School of Pharmacy, Huazhong University of Science and Technology
Xi-Qiu Liu, Associate Professor, School of Pharmacy, Huazhong University of Science and Technology. Dr. Liu received her bachelor degree of Biology in 2007 and doctor's degree of Biomaterials in 2012 at University of Science and Technology of China. After 4 years of Research Fellow at Grenoble INP and CNRS in France, She joined in the School of Pharmacy, Huazhong University of Science and Technology since 2016 to carry out research on bio-fabrication of in vitro disease models for biomedical applications, especially for cancer studies and drug screening. She has published more than 20 high-impact SCI papers such as Advanced Materials, Biomaterials, ACS Applied Materials & Interfaces, Journal of Controlled Release. She also serves as Ambassador of the European Association for Cancer Research.
Cancer development involves a series of changes in cell behaviours and functions, which are mediated by diverse factors in the extracellular matrix (ECM). However, current biological methods are difficult to present those factors (stiffness, growth factor, etc.) simultaneously and dynamically with a tunable manner . Our team has developed certain in vitro cancer models (Figure 1) including hepatocellular carcinoma and osteosarcoma for molecular mechanism studies, based on diverse biomaterial approaches such as layer-by-layer assembly, 3D printing, hydrogels and etc. It was found that the complete EMT process of cancer cells displayed by changing the matrix stiffness or the concentrations of growth factors, and the polarization direction of tumor-associated macrophages could also be controlled by changing the matrix stiffness or viscosity [2-4]. The effective control of the adhesion, morphology and migration of cancer cells was observed by loading specific growth factors, and molecular mechanisms of the combined effect of growth factors and mechanical signaling in the ECM model was clarified on the EMT process and energy metabolism of cancer cells [5-7]. Our research provides a powerful platform to mimic ECM niche, which would open new insights for studies of cancer occurrence and development as well as mechanisms of interactions among cancer cells, ECM and bioactive molecules.
Figure 1. Bio-manufacturing of in vitro tunable cancer models
Acknowledgements: The work was supported by the National Natural Science Foundation of China (81803108, 82072083), Fundamental Research Fund for Central Universities (2018KFYYXJJ023), Natural Science Foundation of Hubei Province (2018CFB113)