Vascularized Micro-organ-on-a-chip based on Microfluidic platforms报告人:
School of Mechatronic Engineering and Automation, Shanghai University
Tao Yue is an Associate Professor in School of Mechatronic Engineering and Automation, Shanghai University. He received the B.S. degree and M.S. degree from Tongji University, Shanghai, China, in 2007 and 2010 respectively, and the Ph.D. degree from Nagoya University, Nagoya, Japan, in 2014. He then worked as a postdoctoral researcher in Ohio State University and University of California, Irvine in US from 2014 to 2018, funded by NSF and NIH. His research interests include Micro/nano robotics, Microfluidic devices and Bio-mems technologies.
Prof. Yue has published more than 40 papers in peer-reviewed journals, including Science Advances、Nano Energy、Advanced Science and Lab on a chip. He has given 15 presentations in international conferences (IEEE ICRA, IROS and MicroTAS). He received the Best Paper Award at the IEEE International Symposium on Micro-Nano Mechatronics and Human Science in 2013 and the IEEE Robotics and Automation Society Japan Chapter Young Award in 2014. He was awarded with Shanghai Young Oriental Scholar in 2017 and Shanghai Science and Technology Committee Rising-Star Program in 2019.
The vascular network of the circulatory system plays a vital role in maintaining homeostasis in the human body. We currently developed a novel modular microfluidic system with a vertical two-layered configuration to generate large-scale perfused microvascular networks in vitro. The two-layer PDMS configuration allows the tissue chambers and medium channels not only to be designed and fabricated independently but also to be aligned and bonded accordingly. This method can produce a modular microfluidic system that has high flexibility and scalability to design an integrated platform with multiple perfused vascularized tissues with high densities. The medium channel was designed with a rhombic shape and fabricated to be semi-closed to form a capillary burst valve in the vertical direction, serving as the interface between the medium channels and tissue chambers. Angiogenesis and anastomosis at the vertical interface were successfully achieved by using different combinations of tissue chambers and medium channels. Various large-scale microvascular networks were generated and quantified in terms of vessel length and density. Minimal leakage of the perfused 70-kDa FITC-dextran confirmed the lumenization of the microvascular networks and the formation of tight vertical interconnections between the microvascular networks and medium channels in different structural layers. This platform enables the culturing of interconnected, large-scale perfused vascularized tissue networks with high density and scalability for a wide range of multi-organ-on-a-chip applications, including basic biological studies and drug screening.