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March, 2022
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Cryogenic 3D printing and the potential applications in tissue engineering






吴斌,华中科技大学材料科学与工程学院讲师、硕士研究生导师,博士毕业于新加坡国立大学。课题组研究方向为3D打印设备软硬件的开发,冷冻打印、同轴打印等3D打印技术在组织工程领域内的应用,成果发表于Bioactive materialsChemical Engineering Journal等生物材料、生物制造领域内知名期刊上。


3D printing has been widely used in tissue engineering to fabricate customized structures. A wide range of biomaterials have been applied. However, there are some requirements for the biomaterials. For example, the materials should be biocompatible and viscous enough for scaffold formation. In this way, some natural materials such as extracellular matrix (ECM) cannot be printed into scaffold shape due to the poor viscosity.

In our previous study, cyrogenic 3D printing was adopted aiming to solve this challenge. Being different from normal 3D printing, a low-temperature substrate was located on the 3D motion platform. ECM slurry was deposited on the cyrogenic platform directly. Hence, the low-viscosity slurry was freezed immediately after printed for formation. Thus, the cryogenic printing provides a broader range of materials to use. In addition, the subsequent lyophilization produces many micro-pores on the fibers, benefiting for cell adhesion and growth. Furthermore, the low temperature provides a friendly environment for cells, and the micro pores could carry different growth factors. These advantages make cryogenic printing a promising technique in tissue engineering.

Figure 1. Cryogenic 3D printing