3D Bio-printing of Multi-functional Dynamic Nanocomposite Bioinks Incorporating Cu-Doped Mesoporous Bioactive Glass Nanoparticles for Bone Tissue Engineering报告人:
朱卉，女，博士，西安交通大学机械学院助理教授。博士毕业于西安交通大学，材料科学与工程专业。博士期间于柏林工业大学联合培养，博士毕业后获得中德（CSC-DAAD）博士后奖学金，于埃尔朗根纽伦堡大学从事博士后研究。主要研究方向包括：生物制造与生物3D打印技术，生物墨水研发及优化，有机/无机生物材料制备，结构功能化设计及组织微环境设计。目前，在Acta Biomaterialia，JMST，Applied Surface Science，Journal of European Ceramic Society 等国内外生物材料领域重要期刊上发表SCI论文10余篇，获授权发明专利1项；担任MDPI审稿委员会常务委员、多次担任知名国际学术期刊如Materials Letters，Ceramics International，Nanomateirals，Molecules等的审稿人。
As a promising technique for bone tissue engineering, bioprinting has seen significant progress in recent years for facilitating the fabrication of bionic tissues with high complexity. However, it remains a challenge to develop cell-laden bioinks in combination with superior physiochemical properties and bio-functionality in favor of bioprinting process and subsequent bone tissue maturation, respectively. In this study, a multi-functional nanocomposite bioink was developed based on amine-functionalized copper-doped mesoporous bioactive glass nanoparticles (ACuMBGNs) and a dynamic hydrogel formulation composed of oxidized alginate and gelatin (ADA-GEL), with favorable rheological properties, improved shape fidelity and structural stability for extrusion-based bioprinting. The reversible dynamic microenvironment in combination with the impact of more cell-adhesive ligands introduced by aminated particles, enables the rapid spreading (within 3 days) and high survival (>90%) of embedded human osteosarcoma cells and bone marrow-derived stroma cells of relevance for bone tissue engineering. Potential osteogenesis and angiogenesis are confirmed in the composite bioprinted constructs likely due to the ion stimulation from the incorporated nanoparticles and cell mechanosensing in the dynamic matrix. In conclusion, we envision that our nanocomposite bioinks could serve as a promising platform for bioprinting complex 3D matrix environments providing superior physiochemical and biological performance for bone tissue engineering.
Figure 1. Scheme of bio-printing using ADA-GEL with aminated mesoporous glass nanoparticles.