毛茅
Engineering of Vascularized Cardiac Tissues with Aligned Architecture Guided by Electrohydrodynamically-printed Microlattices
报告人:毛茅
所在单位:西安交通大学机械工程学院
个人简介:
毛茅，西安交通大学机械工程学院助理教授/博士后，学士、硕士毕业于西安交通大学,获香港理工大学生物医学工程/西安交通大学机械工程双博士，入选国家海外博士后引进计划以及西安交通大学青年优秀人才支持计划A类。主要从事增材制造与生物制造研究，包括仿生血管化组织器官，器官芯片以及高精度功能化生物支架设计与制造等。研究成果在Small, Biofabrication, Acta Biomaterilia等国际期刊发表SCI学术论文10余篇，获2020年度Acta Student Awards奖项，以“生物可降解支架微结构仿生设计制造基础”项目获2019年教育部自然科学一等奖（第6）。曾担任International Journal of Bioprinting特刊编委。
Abstract：
Many native tissues exhibited highly-aligned cellular architectures to maintain unique mechanical and physiological functions. For example, native myocardium consists of multiple layers of aligned cardiomyocytes and the cell orientations among different layers gradually varied, which is important to the systolic mechanics of rotation and twist behaviors as well as the synchronous contraction for efficient blood pumping. Recapitulating such aligned cellular architectures in vitro is important to engineer artificial analogs for desired biological functions.
Here, we proposed a novel strategy to fabricate vascularized cardiac tissues with biomimetic aligned architectures directed by electrohydrodynamically-printed microlattices with predefined microstructures. The cell/ hydrogel, originally filled within the printed microlattices uniformly, was found to gradually develop into densely-populated and highly-aligned bands along the longitudinal direction of the printed microlattices. The presented method was applicable to multiple cell types including primary cardiomyocytes and the gaps formed between the aligned bands and the lateral walls of the microlattice facilitated the subsequent seeding and rapid alignment of other cell types which enables to engineer anisotropic multicellular tissue constructs. The engineered cardiac patches expressed mature cardiomyocyte-specific phenotypes and exhibited synchronous contractive activities. Multilayer cellular alignment with varied orientation in 3D collagen hydrogel was successfully achieved by using electrohydrodynamically-printed microlattices with layer-specific orientations. Vascularized cardiac tissues were successfully engineered and implanted onto the hearts of animals which benefits the beating and pumping function of hearts. This exploration offers a promising way to engineer complex 3D tissue constructs with predefined cellular alignments.
Figure 1. Engineering of highly-aligned cardiac tissues expressing functional proteins
