Construction of Advanced vascularized in vitro tissues via directly multi-cellular organoid printing
Biography:Yuan Pang, Assistant Professor, Department of Mechanical Engineering, Tsinghua University. Dr. Pang received Ph.D. in Bio-engineering from the University of Tokyo in 2013 and did her postdoc fellowship at the Institute of Industry and Science, University of Tokyo in 2013-2015. She started her work as an Assistant Professor at Bio-manufacturing center in Tsinghua University since 2016. Her research interests focus on developing new methods, technologies and explore new applications in the field of biomanufacturing and 3D bioprinting. She aims at in vitro construction of functional micro-organ/tissues, and further use them towards tissue repair, drug delivery and screening, pathology and inflammation study. She has published more than 30 high-impact SCI papers such as Gut, Biomaterials, Biofabrication, Acta Biomaterialia. During her research carrier, she got several scientific awards such as 2020 Young Researcher Award by Lush Prize, Young Scientific Award of International Society of Biomaterials, publication awards of Tissue Engineering and Regenerative Medicine International Society- Asia Pacific. She now serves as the committee member of Chinese Society of Toxicity Testing and Alternatives.
Abstract：The ability to emulate the architecture and function of blood vessels in the integrated context of their associated tissues represents an important requirement for studying a wide range of physiological and disease conditions. Here we reported a methodology for directing rapid assembly of multi-cellular organoids into 3D vascularized tissues with high cellular density and biological performance through 3D bioprinting. By pre-organizing cancer cells and vascular endothelial cells in a customized oxygen-permeable microwell device for 2 days, numerous multi-cellular organoids with uniform size were obtained first. The multi-cellular organoids used as the building blocks were then directly printed together with suspended vascular endothelial cells in hydrogel through extrusion 3D bioprinting to rapidly generate 3D vascularized tissues. The printed in vitro tissue model exhibited a higher level of cell proliferation, hepatic- and endothelial-specific functions, and oxidative phosphorylation efficiency in comparison to conventional printed suspension model. Moreover, the printed multi-cellular tissue model yield significantly more complex and dense vessels, and the cycle for vascularization was shortened (~7 days) in comparison to the printed suspension model. Based on these capabilities, we tested the utilities of current model for investigating the effect of HUVECs on mechanical stability of fabricated tissues, for systematic drug evaluation, and for studying TNFα-induced vascular inflammation.