Acoustic bioassembly for constructing in vitro histotypic and organotypic models报告人:
Prof Pu Chen obtained his BSc in Optical Information Sciences and Technology at the Huazhong University of Sci & Tech (HUST) in 2005. Then, he obtained his Ph.D. degree in Biomedical Engineering at the HUST in 2011. He took postdoctoral training at Harvard Medical School and Stanford School of Medicine from 2011 to 2016. He is currently a full professor at Wuhan University School of Basic Medical Sciences. His research focuses on tissue engineering and organ manufacturing by exploring the power of stem cell biology and engineering. Specifically, he is working on developing organoid-on-a-chip and acoustic bioassembly techniques for constructing human-relevant in vitro histotypic and organotypic models. Now he has published more than 40 papers in the related topics.
Bioassembly is regarded as an alternative approach to bioprinting for 3D biofabrication. Specifically, emerging acoustic bioassembly represents a promising technique to build cell closely packed organotypic constructs. However, simultaneous bioassembly of heterogeneous cell types into a spatially-defined heterocellular architecture remains a long-lasting challenge, but it’s essential to recapitulate native cell organization in natural tissues and organs. In this study, we develop an acoustic differential assembly method to address this critical challenge by locating different cell-containing building blocks to nodal or antinode regions of the Faraday waves based on their inherent physical characteristics. Specifically, cell-containing building blocks are driven by the hydrodynamic drag force and differentially located based on their sizes and buoyant densities. We expect this acoustic differential assembly method will facilitate the construction of HRIOMs with spatially defined heterocellular organization and find wide applications in tissue engineering and regenerative medicine.